relatIonshIp between trace element concentratIons and spermatozoa qualIty In rabbIt semen
نویسنده
چکیده
concentrations of cooper, zinc, iron, manganese, cadmium, lead and nickel in the semen of rabbits (n=27) in relation to spermatozoa quality were evaluated. samples were analyzed on an atomic absorption spectrophotometer (Unicam solar 939). For analysis of pathological spermatozoa semen samples were fixed in Hancock ́s solution and stained with Giemsa dye. For each rabbit at least 500 spermatozoa were evaluated. concentrations of trace elements determined in rabbit semen were following: cooper 20.10±4.09 mg/kg, zinc 81.20±59.43 mg.kg-1, iron 19.65±7.26 mg.kg-1, manganese 30.87±1.81 mg.kg-1, cadmium 0.095±0.10 mg.kg-1, lead 0.092±0.04 mg.kg-1, and nickel 0.297±0.11 mg.kg-1. Total percentage of pathological spermatozoa was 16.21±7.99% with predominance of knob-twisted flagellum, separated flagellum and flagellum ball. Correlations between the copper concentration and acrosomal changes (r=-0.75), total pathological spermatozoa and flagellum ball form (r=0.674) and between total pathological spermatozoa and knobtwisted flagellum (r=0,762) were detected. This study demonstrates that rabbit semen is specific for high concentration of cooper. also a significant effect of zinc on the occurrence of pathological spermatozoa was revealed.
منابع مشابه
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